迟缓爱德华菌的外膜蛋白图谱及耐药性分析

ANALYS OF OUTER MEMBRANE PROTEIN AND DRUG-RESISTANT OF EDWARDSIELLA TARDA

分析 2 7株不同来源的迟缓爱德华菌 (Et)的外膜蛋白 (OMP) ,可分为A～H 8个型。 2 1株致病株与 6株非致病株有明显不同的图谱。致病株OMP条带多而深浓 ,并且相同来源的菌株有几乎一致的图谱。其中国内致病株以E型为主 ,与ATCC参考株相似。非致病株OMP条带则浅而稀疏 ,来源虽不同 ,但图谱极类似。另外 ,6株非致病株对磺胺、庆大、四环素等抗生素普遍耐药 ,而致病株除少数几株外 。

The SDS\|PAGE profiles of out membrane protein(OMP) of 27 Et strains showed diverse patterns and they could be divided into six types. Between 21 pathogenic strains and 6 non\|pathogenic strains there were obviously difference. The pathogenic strains isolated from China belong to E type and were simary to ATCC 15947. The OMP bands of pathogenic strains were more and denser than those of the non\|pathogenic strains, and the strains from same souce had the identical patters. Interestingly, the non\|pathogenic strains from different sources also had the almost same OMP profiles. What\'s more, they were also high resistant to some antibiotics such as Smx, Gem Tet and so on, while the 18 pathogenic strains were senstivity to these.