亚洲玉米螟滞育关联蛋白的分离和纯化

Isolation and purification of diapause-associated proteins from hemolymph in Ostrinia furnacalis

用 6 %聚丙烯酰胺凝胶电泳 ,在亚洲玉米螟Ostriniafurnacalis河南种群滞育幼虫血淋巴中发现了 2种滞育关联蛋白 ,它们的等电点分别为 5 3和 4 7;而在亚洲玉米螟上海种群滞育幼虫血淋巴中发现了 1种滞育关联蛋白 ,等电点为 4 7。亚洲玉米螟河南种群滞育关联蛋白仅在滞育幼虫血淋巴中存在。亚洲玉米螟上海种群滞育关联蛋白在滞育和非滞育幼虫血淋巴中均存在 ,只是它们在滞育幼虫血淋巴中的含量明显高于非滞育幼虫血淋巴中的含量。用 30 %～ 5 0 %丙酮沉淀、Seph aDexG 10 0凝胶过滤和MONOQRH 5 5阴离子交换快速液相法 ,纯化到了高纯度 (≥ 98%)亚洲玉米螟上海种群滞育幼虫血淋巴中的滞育关联蛋白。

By 6% PAGE, it was found that there were two kinds of diapause-associated proteins (DAP)(pI 5 3,4 7) in the hemolymph of diapausing larvae from Henan population and one kind of DAP(pI 4 7) in the hemolymph of diapausing larvae from Shanghai population of Ostrinia furnacalis. The DAPs of O. furnacalis from Henan population were found only in the hemolymph of diapausing larvae. Although the DAP of O.furnacalis from Shanghai population was found in the hemolymphs of both non-diapausing and diapausing larvae, it had higher titer in diapausing larvae. By means of 30%～50% acetone fractionation,Sephadex G-100 gel filtration chromatography and MONO Q RH 5/5 FPLC, the high pure DAP(≥98%) from the hemolymph of larvae from Shanghai population was isolated.