用微卫星引物PCR分析棉蚜不同蚜型的DNA多态性

Microsatellite DNA polymorphism in different forms of the cotton aphid

用微卫星引物PCR方法 ,对棉蚜Aphisgossypii干母、干雌、孤雌蚜 (有翅迁飞孤雌蚜和无翅孤雌蚜 )、性母、性雌蚜和雄蚜的DNA多态性进行分析。结果表明 :①棉蚜的有性蚜型和无性蚜型之间在遗传上有较大的差异 ;②性母与性雌蚜和雄蚜的遗传关系都十分接近 ,说明同一性母既产性雌蚜 ,也产雄蚜 ;③干母和干雌的遗传关系很近 ,但孤雌蚜已与二者有分化。

The PCR technique with microsatellite primers was used to analyze the DNA polymorphism in different forms (mother of mating forms, mating female, male, fundatrix, apterous and alate virginoparae ) of the cotton aphid, Aphis gossypii. The results showed that: 1. There existed a obvious genetic differentiation between sexual forms and parthenogenetic forms of the cotton aphid;2. Mother of mating forms had almost the same genetic relation to the mating female and male, suggesting that the mother of mating female also be the mother of male;3. Compared with virginoparae, the genetic relationship between fundatrix and the first generation of virginoparae (apterous) produced by fundatrix (apterous) was much close.