BT型细胞质雄性不育水稻及其三系的线粒体DNA研究

RAPD analysis for mitochondrial DNAs of the BT cytoplasmic male sterile (cms) line and its associated lines in rice (Oryza sativa)

用 RAPD技术对 BT型水稻胞质雄性不育系秀 A及其保持系秀 B、恢复系湘晴以及杂种 F1代的线粒体 DNA进行了比较分析。结果表明不育系与其保持系间存在显著差异 ;不育系与其 F1之间 mt DNA也存在差异。在引物 OPJ- 0 8的扩增产物中 ,秀 A扩增出一条分子量为 80 0 bp的多态性片段 ;在引物 OPK- 1 0的扩增产物中 ,杂种 F1扩增出一条分子量为 90 0bp的片段。把这两片段回收、克隆并制备探针 ,OPJ- 0 880 0 的 Southern杂交结果显示不育系与其 F1杂交图谱存在多态性 ;OPK- 1 0 90 0 的 Southern杂交结果显示不育系与其保持系间存在差异。

BT type rice cytoplasmic male sterility line were compared with its maintainer,restorer and F 1 hybrid using mitochondrial DNA (mtDNA) RAPD analysis.The results showed that difference was between not only the cms line and its maintainer,but also cms line and its F 1 hybrid.Primer OPJ-08 generated a polymorphic product of 800 bp in sterile line,and primer OPK-10 amplified a specific fragment of 900 bp in F 1 hybrid.The two fragments were then cloned into plasmid vector and converted to RFLP probes.Southern hybridization patterns revealed polymorphism between sterile line and F 1 hybrid using OPJ-08 800 fragment as probe,and between sterile line and maintainer line using OPK-10 900 fragment as probe.We infered that the two mtDNA fragments may be associated with cms in rice.