β-TCP降解和rhBMP-2诱导成骨的关系(英文)

RELATIONSHIP BETWEEN DEGRADATION OF β-TCP AND OSTEOGENESIS INDUCED BY rhBMP-2

探讨β -TCP的降解率是否可以影响rhBMP -2的诱导成骨量。将不同降解率的β -TCP1和β-TCP2分别与rhBMP -2复合制备成不同降解率的两种复合物 ,β -TCP1 /rhBMP -2和β -TCP2 /rhBMP -2。然后分别种植于小鼠股部肌袋中。种植后分别进行组织学检查、组织形态计量、碱性磷酸酶和降解量测定。两种复合物种植后均有软骨和骨形成 ,成骨量随时间推移而增加。但是 ,种植后 8周时β-TCP1 /rhBMP -2组成骨量显著高于β-TCP2 /rhBMP -2组 (P <0 .0 5)。种植后 4周以前两组的ALP水平均随时间推移而升高 (P <0 .0 5) ,但是 ,在 4～ 8周间则无明显差异。此外 ,种植后 8周时β -TCP1 /rhBMP -2的降解量显著高于β -TCP2 /rhBMP -2 (P<0 .0 5)。β -TCP的降解率可以影响BMP的诱导成骨量。

To determine if degradation rate of β-TCP may influence the amounts of bone formation induced by rhBMP-2. Two kinds of β-TCP/rhBMP-2 composites(β-TCP1/rhBMP-2and β-TCP2/rhBMP-2) which own different degradation rate,were fabricated by combining β-TCP1 and β-TCP2 with rhBMP-2,and implanted into the muscle pouches in the thigh of mice.Histological study,histomorphometry,assay of alkaline phosphatase and degradation amounts of two kinds of β-TCP/rhBMP-2 were performed after implantation. Both of β-TCP1/rh BMP-2 and β-TCP2/rhBMP-2 composite implants showed signs of cartilage and bone formation.The amounts of new bone were increasing with time.But the amounts of new bone in β-TCP1/rhBMP-2 implants were much greater than in β-TCP2/rhBMP-2 implants at 8-week after implantation(P<0.05).The levels of ALP activity in both groups were increasing with time before 4 weeks postimplantation(P<0.05),but there was no change during 4～8 weeks.Besides,biodegradation rate of β-TCP1/rhBMP-2 was higher than that of β-TCP2/rhBMP-2 at 8-week after implantation(P<0.05). The degradation rate of β-TCP can influence the amounts of BMP-inducing bone formation.