国产“水山姜”的分类学研究——来自核糖体DNAITS区序列的证据

Studies on Systematics of "Alpinia aquatica " from China: Evidence from ITS Sequences of Nuclear Ribosomal DNA

用直接测序法对国产黑果山姜Alpinianigra (Gaertn.)Burtt以及“水山姜Alpiniaaquatica(Koen.)Rosc .”的核糖体DNA中的内转录间隔区 (ITS)序列进行了测定 ,结果显示两者序列完全一致 ;ITS 1长度为 1 78bp,ITS 2长度为 2 32bp,5 8S编码区长度为 1 64bp ,GC含量为56 9%。形态学特征结合DNA分子证据 ,认为《中国植物志》

Nuclear ribosomal DNA internal transcribed spacer (ITS) regions of Alpinia nigra (Gaertn.) Burtt and 'Alpinia aquatica (Koen.) Rosc.' from China were directly sequenced using an automated sequencer. The result showed that both sequences were identical. The size is 178 bp in ITS 1, 232 bp in ITS 2 and 164 bp in 5.8S coding region, G+C content being 56.9%. According to the morphological and DNA characters, it was suggested that 'Alpinia aquatica (Koen.) Rosc.' recorded in Flora of China should be Alpinia nigra (Gaertn.) Burtt.