培养基和培养条件对栀子悬浮细胞合成多糖的影响

Effects of Medium and Culture Conditions on Polysaccharide Synthesisby Suspension Cell Culture of Gardenia jasminoides Eills

对栀子悬浮细胞合成多糖的调控因子研究表明 :B5为最适培养基 ;5～ 10d继代周期的细胞可以保持良好的生长状态和多糖的合成能力 ;80g L的鲜细胞的接种量有利于栀子细胞的生长和多糖的合成 ;使用单一碳源时 ,葡萄糖比蔗糖对细胞生长更有益 ,但葡萄糖成本高 ,因而混合碳源 4 5g L(葡萄糖 :蔗糖 =1∶1)是最佳配方 ;氮源种类对细胞生长和多糖合成没有明显的影响 ,但氮源浓度是主要因素 ,4 0～ 5 0mmol L是最佳浓度 ,同时运用悬浮细胞生产栀子多糖可以通过在不同时间收获的细胞来避免提取时黄色素的干扰 ,具有很好的实际意义。

The effects of medium and culture condition on polysaccharide synthesis by suspension culture of %Gardenia jasminoides% Eills were studied.The results show that B5 was the optimum medium,5～10 days was suitable for subculture periods,and an inoculum of 80g/L wet cell was better to cell growth and polysaccharide accumulation.For carbon source,it was better to use glucose than sucrose on cell growth,but due to the higher price of glucose,45g/L compound carbon source combined sucrose with glucose (1∶1) was the optimum.The effect of different kinds of nitrogen source on cell growth and polysaccharide synthesis was not so big,and 40～50mmol/L nitrogen was optimum,lower concentration of nitrogen source could inhibit the synthesis of polysaccharides.In addition,by controlling the harvest time of the polysaccharide in the suspension culture,the accumulation of yellow pigment could be prevented,which is easier to the polysaccharide purification.