期刊文献+
任意字段
题名或关键词
题名
关键词
文摘
作者
第一作者
机构
刊名
分类号
参考文献
作者简介
基金资助
栏目信息

甾体化合物RSA的11β-羟基化反应 被引量：2

Compound RSA 11β-Hydroxylation with Curvularia lunata
下载PDF
导出
摘要 Compound RSA 11β-Hydroxylation of %Curvularia lunata% was studied.The composition of hydroxylation products was analyzed.It was found that the conversion rate with protoplasts was increased conspicuously,the construction and quantity of hydroxylation products was not alteration,but the percentage was changed greatly.The 14α-OH percentage of protoplasts was 3.03 -fold than that of mycelia, the 11α-OH percentage was decreased 60%,and the conversion ratio of 11β-OH was more 1.29-fold than that of the mycelia.It affected the percentage of hydroxylation products when 2 mg/mL polyoxins were added into the mycelia conversion system. Compound RSA 11β-Hydroxylation of %Curvularia lunata% was studied.The composition of hydroxylation products was analyzed.It was found that the conversion rate with protoplasts was increased conspicuously,the construction and quantity of hydroxylation products was not alteration,but the percentage was changed greatly.The 14α-OH percentage of protoplasts was 3.03 -fold than that of mycelia, the 11α-OH percentage was decreased 60%,and the conversion ratio of 11β-OH was more 1.29-fold than that of the mycelia.It affected the percentage of hydroxylation products when 2 mg/mL polyoxins were added into the mycelia conversion system.
作者 王敏 路福平 王风清 蒋岳 杜连祥
机构地区 天津轻工业学院食品工程系
出处 《生物工程学报》 CAS CSCD 北大核心 2001年第6期710-712,共3页 Chinese Journal of Biotechnology
关键词 11β-羟基化 新月弯孢霉 原生质体 甾体化合物 RSA protoplasts,11β-Hydroxylation, Curvularia lunata, compound RSA
分类号 Q591 [生物学—生物化学]
  • 相关文献

参考文献5

二级参考文献12

  • 1张峻,邢来君,王红梅.γ-亚麻酸高产菌株的选育及发酵产物的分离提取[J].微生物学通报,1993,20(3):140-143. 被引量：41
  • 2钟辉,张峻,邢来君.微生物发酵法生产γ－亚麻酸的研究进展[J].微生物学通报,1994,21(4):237-239. 被引量：34
  • 3徐诗伟.微生物转化在药物合成中的应用前景[J].中国医药工业杂志,1996,27(9):422-430. 被引量：18
  • 4邢来君，真菌学报，1987年，6卷，4期，242页
  • 5梁平彦，植物生理学报，1981年，7卷，1期，1页
  • 6广州第八制药厂中试室，医药工业杂志，1980年，8期，3页
  • 7R. Komel,D. Rozman,A. Puc,H. So?i?. Effect of immobilization on the stability ofClaviceps purpurea protoplasts[J] 1985,Applied Microbiology and Biotechnology(2):106～109
  • 8Leon Sedlaczek,Jerzy D?ugoński,Adam Jaworski. Transformation of steroids by fungal protoplasts[J] 1984,Applied Microbiology and Biotechnology(3):166～169
  • 9L. Ferenczy,F. Kevei,M. Szegedi. High-frequency fusion of fungal protoplasts[J] 1975,Experientia(9):1028～1030
  • 10L. Ferenczy,F. Kevei,M. Szegedi. Increased fusion frequency ofAspergillus nidulans protoplasts[J] 1975,Experientia(1):50～52

共引文献31

同被引文献18

  • 1郭一平,郑璞.甾体微生物C_(11)α-羟化反应的研究进展[J].浙江工业大学学报,2004,32(4):437-441. 被引量：9
  • 2曾本秀,陈树群,陈伯林.提高甾体微生物11β-羟化的酶活性[J].中国医药工业杂志,1993,24(12):529-532. 被引量：13
  • 3林新国,周才赋.梨头霉菌生产11β羟基化化合物RS－21醋酸酯的研究[J].化学反应工程与工艺,1995,11(2):213-216. 被引量：5
  • 4Wilmanska D,Milcazarek K,Rumijiowska A,et al.Elimination of by-products in 11β-hydroxylation of substance S using Curvularia lunata clones regenerated from NTG-treated protoplasts[J].Appl Microbiol Biotechnol,1992,37 (5):626-630.
  • 5Paraszkiewicz K,Dlugonski J.Cortexolone 11β-hydroxylation in protoplasts of Curvularia lunata[J].J Biotechnol,1998,65(2-3):217-224.
  • 6杨顺楷,肖勇,赖涛,等.微生物甾体转化生产氢化可的松加工过程研究的新进展[D].中国科技网通讯,成都:中国科学院成都生物研究所,2003.6.
  • 7Chincholkar GV,Sukhodolskaya GV,Baklashora TG,et al.Peculiarities of the 11 β-hydroxylation of steroid compound S by the mycelium of Curvularia lunata BKM F-644 in the presence of β-cyclodextrin[J].Prikl Biokhim Microbiol,1992,28 (5):685-693.
  • 8Chen KC,Wey HC.11β-hydroxylation of coxtexolone by Curvularia lunata[J].Enzyme Microb Technol,1990,12 (4):305-308.
  • 9杨顺楷,肖勇,赖涛,等.微生物甾体转化生产氢化可的松加工过程研究的新进展[D].中国科技网通讯,.
  • 10张丽青.微生物转化在甾体药物合成中的应用[J]医药工业,1985(01).

引证文献2

二级引证文献7

生物工程学报
2001年 第6期

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部