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改良的乳猪肝细胞微载体粘附培养方法

Modified Microcarrier Adherent Culture Method of Porcine Hepatocytes
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摘要 目的:改进常用的肝细胞手工震荡微载体粘附培养技术,提高肝细胞粘附率。方法:用磁力悬浮培养容器使肝细胞轻度聚集,然后加入微载体Cytodex-3培养,在控制低转速的情况下使肝细胞更易粘附在微载体上。结果:磁力搅拌粘附培养的肝细胞粘附率高(90.3%±7.7%)且较稳定(变异系数:8.6%).明显忧于手工震荡粘附培养(粘附率:69石%±18.3%,变异系数:26.4%,P<0.005);前2周内的白蛋白合成功能亦强于手工震荡粘附培养(P<0.05)。结论:磁力搅拌粘附培养肝细胞粘附宰高、重复性强、稳定性好、操作方便,且减少了肝细胞受污染的机会。 Background/Aims: To modify and optimize the method for cuhivation of porcine hepatocytes on microcarriers and to increase the rate of adhesion of hepatocytes. Methods: Magnetic suspension cul- ture vessel was utilized for hepatocytic formation of small spheroids, then microcarrier C todex-3 was added. Under the influence of magnetic force, porcine hepatocytes became easier to adhere to the ml- crocarriers at a low rotational speed. Results: The adhesion rate of the new method was significantly higher (90.3%±7.7%) and more stable [coefficient of variation (CV): 8.6%] than that through hand stir- ring (adhesion rate: 69.5%±18.3%, CV: 26.4%. P<0.005), the albumin synthesized within two weeks was also higher in the former (P<0.05). Conclusions: This modified method has the advantages of higher adhesion rate, good repetition, stabilization and easier to operate, which also can reduce the chance of contamination.
出处 《胃肠病学》 2001年第3期141-143,共3页 Chinese Journal of Gastroenterology
关键词 细胞粘附 细胞培养 磁力学 肝细胞移植 Cell Adhesion Cell Culture Magnetics
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参考文献3

  • 1王英杰,李梦东,王宇明,王小红,聂青和.人肝细胞的微载体培养及其意义[J].中华消化杂志,1997,17(4):191-194. 被引量:8
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二级参考文献4

  • 1王英杰,中华肝脏病杂志,1996年,4期,178页
  • 2王宇明,中华医学杂志,1995年,75卷,437页
  • 3鄂征,组织培养和分子细胞学技术,1995年,109页
  • 4王宇明,中华消化杂志,1994年,14卷,175页

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