内镜逆行胰胆管造影前后血清肿瘤坏死因子-α的检测及其临床意义

Comparison of Serum Tumor Necrosis Factor-α Concentration between Pre- and Post-endo-scopic Retrograde Cholangiopancreatography and its Clinical Significance

目的：探讨内镜逆行胰胆管造影（ＥＲＣＰ）前后血清肿瘤坏死因子（ＴＮＦ）－α含量变化与胰腺损伤的关系。方法：用放射免疫法测定２６例患者行ＥＲＣＰ前后的血清ＴＮＦ-α 水平，用Ｗａｌｌｆｅｌｓ法测定血清淀粉酶水平。结果：ＥＲＣＰ术后２ｈ，血清淀粉酶及ＴＮＦ－α值最高；血清淀粉酶显著升高者ＴＮＦ-α升高亦较显著（Ｐ＜０．０５）；２例发生急性胰腺炎的患者应用生长抑素制剂后，ＴＮＦ－α水平显著下降。结论：ＴＮＦ－α参与了胰腺损伤的发生。

Background/Aims: To detect the serum tumor necrosis factor (TNF)-α before and after endoscopic retrograde cholangiopancreatography (ERCP) and to appraise their relevance with the pancreatic in- jury. Methods: The serum TNF-α level was determined it-i 26 patients with ERCP using radioimniuno- assay, and the serum amylase level was detected by Wallfels method. Results: The serum amylase and TNF-α reached their peaks two hours after ERCP; in patients with increased serum amylase, the TNF- α level also increased significantly (P< 0.05), but decreased after the use of somatostatin in 2 patients with acute pancreatitis. Conclusions: TNF-α is involved in the pathogenesis of pacreatic injury.