人表皮细胞与无细胞异种真皮复合移植的实验研究

An experimental study on the recombinant composite skin graft consisting of human epithelium and acellular xeno-dermis

目的 观察人表皮细胞与无细胞异种真皮复合移植全层皮肤缺损创面后的效果 ,寻找一种新的创面覆盖物。 方法 42只裸鼠作背部全层皮肤缺损创面 ,分别进行复合皮移植 (复合皮组 )和单纯表皮细胞膜片移植 (对照组 ) ,术后定期观察创面愈合情况并进行创面愈合率及创面收缩率的计算 ,同时留取创面组织进行组织学检测。 结果 与对照组相比较 ,复合皮组的创面愈合及外观情况良好 ,两组创面愈合率及收缩率的差异有显著性意义 (P <0 0 5 ) ;组织学检测提示复合皮上皮分化充分 ,胶原增生有序 ,表皮 真皮连接结构重建明显 ,未见明显的急性期免疫排斥反应。结论 人表皮细胞与无细胞异种真皮复合移植能改善创面愈合质量 ,可作为一种新的皮肤代用品。

Objective To observe the effects of th e recombinant composite skin graft consisting of human epithelium and acellular xeno-dermis on full thickness skin loss wounds,so as to find a new wound coveri ng material. Methods Forty-two nude mice with full thickness skin loss on the back were grafted with the composite skin (compound skin grafting group,G) a nd human keratinocyte sheet(control group,C),respectively.The wound healing cond itions,such as wound healing rate and wound contraction rate,were observed at di fferent time points after the operation.Simultaneously,wound tissue samples were harvested for histological examination. Results The wound healing rate in G group was much higher than t hat in C group.In contrast,the wound contraction rate in G group was obviously l ower than that in C group(P<0.05).It was indicated by histological examinat ion that there was full differentiation of epithelium,orderly collagen prolifera tion and obvious reconstruction of the epithelial-dermal conjunction structure in G group.There was no obvious sign of acute immune rejection. Conclusion The wound covering with recombinant composite skin g raft of human epithelium and acellular xeno-dermis could improve wound healing quality.The composite skin could be a new skin substitute.