L-精氨酸预防大剂量顺铂所致家兔急性肾毒性的实验研究

The experimental study of L - arginine preventing HD - PDD - induced acute nephrotoxicity in rabbits

目的:观察L-精氨酸预防大剂量顺铂所致的家兔急性肾毒性的疗效。方法:2～3kg的家兔16只,雌、雄各半,随机分为4组（亦雌、雄各半）:A组为对照组;B组为单用顺铂组,C组为顺铂联合高剂量L-精氨酸组（1.3g/kg）,D组为顺铂联合低剂量L-精氨酸组（0.5g/kg）,顺铂用量均为6mg/kg,用药后72小时处死家兔取其肾脏观察病理变化。结果:A组正常;B组区域性肾小管上皮细胞坏死、脱落,坏死的肾小管内充满大量的坏死物及蛋白管型;C组未见肾小管上皮细胞坏死、脱落,管腔内偶见蛋白管型,D组零星区域肾小管上皮细胞坏死、脱落,管腔内见多量坏死物及蛋白管型。结论:L-精氨酸可有效预防顺铂所致的家兔急性肾毒性,且以高剂量（1.3g/kg）为优。

Objective: To observe the preventive effect of L-arginine on the HD-PDD induced acute phrotoxicity in rabbits. Methods: Each of 16 rabbits weight 2-3kg, female:8, male:8, All rabbits were divided into four groups randomly, each group included two males and two females, group A was the control group, group B was FDD - alone group, group C was FDD combined high -does L-arginine (1. 3g/kg), group D was FDD combined low-dose arginine(0. 5g/kg) . The dose of FDD was 6mg/kg. One of each group rabbits was killed and kidneys were cut after injecting drugs 72 hours later; then pathologic changes was observed. Results: Group A: normal; group B: proximal tubule epithelial cells necrosis and falling were observed in large amount of cells, necrosis matters and tube-shape protein were filled in the proximal tubule of necrosis; group C: there were no proximal tubule epithelial cells necrosis and falling, tube - shape protein were observed occasionally; group D: proximal tubule epithelial cells necrosis and tube-shape protein were observed. Conclusion; L-arginine can significantly prevent the HD?FDD induced acute nephrotoxicity in rabbits and 1.3g/kg group is superior.