粉防己碱对瘢痕成纤维细胞DNA和胶原合成的影响

Effects of tetrandrine on the synthesis of collagen and scar-derived fibroblast DNA

目的 探讨粉防己碱对瘢痕成纤维细胞ＤＮＡ和胶原合成的影响。方法 以体外培养的人瘢痕成纤维细胞为实验模型，将粉防己碱（５－８０ ｍｇ／Ｌ）加入细胞培养液中进行干预并与对照组比较，用３Ｈ－ＴｄＲ掺入法和３－脯氨酸掺入法分别测定各组瘢痕成纤维细胞３Ｈ－ＴｄＲ掺入值与３Ｈ脯氨酸掺入值，以反映其ＤＮＡ和胶原合成水平的变化。结果 粉防己碱预处理浓度由５ ｍｇ／Ｌ升至８０ ｍｇ／Ｌ时：（１）瘢痕成纤维细胞３Ｈ－ＴｄＲ掺入值（ｍｉｎ－１）由对照组的１７４０ ± １６５分别降至１１６２ ±２２６、４１２±８２，抑制率达７６．３２％，组间差异有非常显著意义（Ｐ＜０．０１）；（２）瘢痕成纤维细胞３Ｈ－脯氨酸掺入值（ｍｉｎ－１）由对照组的１１２６ ± １９３分别降至５３５± １４１、３４１± ８９，抑制率达６９．７１％，组间差异有非常显著意义（Ｐ＜０，０１）。结论 粉防己碱对体外培养的瘢痕成纤维细胞ＤＮＡ和胶原合成具有抑制作用，呈剂量依赖关系，具有防治增生性瘢痕的应用前景。

Objective To investigate the effects of tetrandrine on the synthesis of collagen and scar- derived fibroblast DNA. Methods Scar-derived human fibroblasts were cultured in vitro. The changes in the levels of the synthesis of collagen and DNA of the fibroblasts were represented by the incorporation values of 'H-TdR and 3H-proline into the cells. Tetrandrine was added to the culture media of the cells,and its effects were studied. Results When the concentration of added tetrandrine increased from 5mg/L to 80mg/L, The 3H-TdR values in scar-derived fibroblasts were 1162 ±226 and 412 ± 82, respectively while that in con- trol group was 1740 ± 165 .showing an inhibition rate of 76. 32% , and the difference( P < 0. 01 ) . In addi- tion, the 3H-proline incorporation values in the cells were 535 ± 141 and 341 ± 89 , respectively, while that in control group was 1126 ± 193 ,with the inhibition rate of 69. 71% , showing signficant difference ( P < 0. 01). Conclusion The synthesis of DNA and collagen in cultured scar-derived fibroblasts could be inhibi- ted by tetrandrine in dose-dependent pattern. Tetrandrine might be a potential agent for the prevention and treatment of proliferative scars.