枸杞多糖抑制人白血病细胞生长的研究

Inhibition the growth of human leukemia cells by Lycium barbarum polysaccharide

用体外培养法研究枸杞多糖 (LBP X)对人白血病HL 6 0细胞生长的抑制作用并探讨其作用机制。结果表明 :LBP X(2 0、10 0、5 0 0和 10 0 0mg L)与HL 6 0细胞共培养 48h后 ,LBP X抑制HL 6 0细胞增殖呈剂量依赖性关系 ,且可降低HL 6 0细胞的膜流动性 ,HL 6 0细胞的DNA琼脂糖凝胶电泳可见明显的“DNAladder” ,TUNEL方法检测呈阳性。这提示诱导HL 6 0细胞凋亡是LBP

The effect and the mechanism of Lycium barbarum polysaccharide (LBP X) on inhibiting the growth of human leukemia HL 60 cells were examined. LBP X(20,100,500,1000 mg/L) could inhibit the growth of HL 60 cells in dose dependent manner and decrease the membrane fluidity of the cell. Agarose gel electrophoresis of DNA from the cells treated with LBP X revealed a “DNA ladder” and positive TUNEL test. The results showed that the apoptosis of HL 60 cells induced by LBP X maybe its important mechanism on anti tumorgenesis.