反义TGF-β_1抑制瘢痕疙瘩成纤维细胞增殖的研究

An experimental study of antisense TGF-β_1 inhibiting keloid fibroblast proliferation in vitro

目的 应用反义技术抑制瘢痕疙瘩成纤维细胞 (KF)体外增殖 ,为应用基因治疗手段改善创伤愈合提供实验依据。方法 应用脂质体将反义TGF β1 转染至KF ,通过细胞计数绘制细胞生长动力学曲线 ,流式细胞仪检测细胞的凋亡情况。结果 ①转染反义TGF β1 的KF体外增殖明显降低。②与未转染反义TGF β1 的KF相比 ,转染反义TGF β1 的KF凋亡发生率明显增高 (差异有显著性意义 ,P <0 0 5 )。结论 反义TGF β1 可抑制体外培养的KF增殖 。

Objective\ To study the effect of antisense TGF β 1 on keloid fibroblast proliferation in vitro. Methods\ The antisense TGF β 1 was trasfected into the keloid fibroblast by liposome. The cell count technique was used to test the effect of antisense TGF β 1 on keloid fibroblast proliferation in vitro. Cell apoptosis was tested by FCM. Results\ Antisense TGF β 1 can inhibit the proliferation of keloid fibroblast in vitro; Antisense TGF β 1 can increase the apoptosis ratio of keloid fibroblast in vitro. Conclusions\ Antisense TGF β 1 can induce keloid fibroblast apoptosis and inhibit keloid fibroblast proliferation in vitro, which may contribute to the inhibition of keloid formation in vivo.