异丙酚对原代培养海马神经元缺氧反应的影响

Effects of propofol on the anoxic response of primary cultured hippocampal neurons

目的 观察不同浓度异丙酚对原代培养海马神经元缺氧反应的影响。方法 培养 12d海马神经元 ,随机分为四组 :正常对照组、缺氧组、缺氧加异丙酚 5 0 μmol L组 ,缺氧加异丙酚 5 0 0 μmol L组。测定乳酸脱氢酶 (LDH)漏出率及c fos免疫组化。结果 位于细胞核内的c fos阳性蛋白缺氧前染色为浅紫色 ,缺氧后呈紫黑色且阳性细胞数量增加 (P <0 0 1) ,而加入异丙酚后能减少这种反应。缺氧组LDH漏出率较正常组增加 (P <0 0 1) ,异丙酚组能降低缺氧组增加的LDH漏出率 (P <0 0 5 ) ,且与异丙酚剂量有关。结论 异丙酚能提高体外海马神经元的缺氧耐力 ,减少LDH漏出率和c fos蛋白过度表达。

Objective To evaluate the effects of different concentration of propofol on the anoxic response of primary cultured hippocampal neurons Methods Newborn (<24h) Wistar rats were used Hippocampal neurons having been cultured for 12 d were randomly divided into four groups: control group, anoxia group, propofol 50μmol/L group , anoxia+propofol 500μmol/L group Lactic dehydrogenase(LDH) efflux ratio was assayed and c fos protein immunohistochemical staining were performed in every group Results The c fos protein expression in propofol groups was significantly less than that in anoxia group LDH efflux ratio significantly increased in anoxia groups(P<0 01) as compared with that in control group Propofol reduced the increase in LDH efflux induced by anoxia in a concentration dependent manner Conclusions Propofol can enhance the tolerance of anoxia in primary cultured hippocampal neurons by decreasing LDH efflux ratio and c fos protein expression