异丙酚对大鼠离体心脏缺血再灌注损伤的保护作用

Protective effects of propofol on isolated rat heart against ischemia-reperfusion injury

目的 观察异丙酚对大鼠离体心脏缺血 再灌注损伤时左右心室功能的影响。方法 雄性SD大鼠 16只分成对照和实验组 ,行Langendorff灌注后 ,刺激器控制心率在 348次 min ,均经历平衡期 2 5min、全心缺血期 30min和再灌注期 4 0min。实验组在平衡的后半期持续灌注含有异丙酚6 μg ml的灌注液 10min。灌注期间通过心室内的乳胶水囊经换能器连于MacLabInstrument和计算机 ,观察两组左右心室等容收缩时压力和速度指标的变化。定时测量冠脉流量。再灌注末取左室心肌组织测定心肌SOD活性。结果 再灌注后 ,实验组的LVDP和RVDP明显上升 ,而LVEDP和RVEDP显著降低 ;同时左右心室dp dtmax较对照组显著增高 ,而dp dtmin 显著降低。再灌注期实验组的冠脉流量增加。再灌期末实验组心肌组织SOD活性较对照组显著升高。结论 缺血前灌注 6 μg ml异丙酚对大鼠离体心脏缺血再灌注损伤时左右心室的舒缩功能和心肌的内在收缩性具有一定的保护作用。异丙酚增加了再灌注期的冠脉流量 ,提高了心肌组织的SOD活性。

Objective To assess the effects of propofol on left and right ventricular function of isolated rat heart against ischemia reperfusion injury Methods Sixteen male SD rats were randomly divided into control and experiment groups The isolated rat hearts was connected to Langendorff preparation and perfused as in our previous experiment After being perfused for 25 min, the isolated heart was subjected to 30 min no flow global ischemia followed by 40 min reperfusion The temperature of the isolated heart was maintained at 36℃ 37℃ during global ischemia In experiment group the isolated heart was perfused with propofol 6μg/ml in perfusate for 10 min before global ischemia The heart rate was paced at 348 beats/min The isovolumetric force velocity indexes and coronary flow were monitored continuously with MacLab instruments Results As compared with the isolated hearts in control group, propofol (6μg/ml) perfusion before ischemia significantly improved left and right ventricular diastolic function by decreasing ventricular end diastolic pressure, dp/dt min and T value At the same time, propofol protected left and right ventricular systolic function by elevating developed pressure, dP/dtmax and Vpm during reperfusion At the end of reperfusion, ventricular tissue superoxide dismutase (SOD) activity was significantly well preserved in the hearts pretreated with propofol Conclusions Propofol 6μg/ml perfusion before ischemia protects the isolated rat heart against ischemia reperfusion injury by improving diastolic and contracting function of right and left ventricles and intrinsic contractivity of myocardium Propofol increases coronary blood flow during reperfusion and increases SOD activity of myocardial tissue