国产司帕沙星体外抗菌作用研究

Study in vitro of antimicrobial activity of domestic sparfloxacin

目的 比较司帕沙星 (SPLX)与洛美沙星 (LM LX)、环丙沙星 (CPLX)、氧氟沙星 (OFLX)和依诺沙星 (ENX)的体外抗菌活性。方法 采用琼脂平板稀释法测定MIC ,以NCCLs标准判读敏感性。结果 SPLX对受试菌的抗菌活性最强 ,MIC90为 2mg·L- 1,而LMLX、CPLX、OFLX和ENX的MIC90 分别为 16、16、16和 32mg·L- 1。SPLX的MIC几何均数 (MICg)显著低于LMLX、OFLX和ENX ,略低于CPLX。SPLX对革兰氏阳性球菌的敏感率为 10 0 % ,明显高于其它对照药物。结论 SPLX比其他氟喹诺酮类抗菌谱扩大。

Aim To study in vitro the antibacterial activity of domestic sparfloxacin(SPLX) and compare it with other four quinolones including lomefloxacin(LMLX), ciprofloxacin(CPLX), ofloxacin(OFLX) and enoxacin(ENX). Methods Minimal inhibitory concentrations(MIC) were determined by means of agar disk dilution method. The susceptibility was defined according to NCCLs. Results SPLX was the most active one in the quinolones against all tested strains. Its MIC 90 was less or equal to 2 mg·L -1 , while the MIC 90 of LMLX?CPLX?OFLX and ENX were 16?16?16 and 32 mg·L -1 , respectirely. The geometric means of MIC(MIC g) of SPLX (0.179 mg·L -1 ) were significant lower than MIC g of LMLX?OFLX and ENX and slightly lower than that of CPLX. The susceptible rate of G + cocci was 100%, significantly higher than that of controlled quinolones. Conclusion The antibacterial spectrum of SPLX is wider and the activity against clinical isolates is higher than that of other quinolones.