垂体腺瘤bFGF表达、血管形成与侵袭性的关系

The relationship between the expression of basic fibroblast growth factor, angiogenesis and invasiveness of pituitary adenoma

目的：探讨碱性成纤维细胞生长因子 ( bFGF )表达、肿瘤血管形成在垂体腺瘤侵袭性中的意义. 方法：用免疫组化法(SABC法)检测 39例侵袭性垂体腺瘤、19例非侵袭性垂体腺瘤和4例正常垂体腺组织中 bFGF的表达,并用第Ⅷ因子相关抗原(F8-RA)免疫组化染色来计数垂体腺瘤的血管数量(微血管密度),探讨二者与肿瘤侵袭性的关系. 结果：bFGF的表达、肿瘤血管形成与垂体腺瘤的侵袭性密切相关.侵袭性垂体腺瘤bFGF表达水平、血管计数显著高于非侵袭性垂体腺瘤和正常垂体腺组织,而非侵袭性垂体腺瘤与正常腺组织间则无显著差异(t=1.469,P>0.05)；侵袭性腺瘤中bFGF表达水平与肿瘤微血管数量呈正相关(r=0.361,P<0.05). 结论：bFGF可能通过促血管生成作用刺激垂体腺瘤生长与侵袭.

Objectives:To study the relationship between the ex p ression of basic fibroblast growth factor (bFGF), angiogenesis and invasiveness of pituitary adenoma. Methods:The protein expressions of bFGF, f actorⅧ were examined by immunohistochemical SABC methods in 39 invasive pituit ary adenomas and 19 noninvasive adenomas. Image analyzer system (Eagle-EyeⅡ) was used to determine the expression levels of bFGF. Results：Hig her expression of bFGF was found in invasive pituitary adenomas which was mostly expressed in cell plasma and the vascular wall. There was a significant correla tion among bFGF expression, microvascular density and the invasiveness of pituit ary adenomas. Conclusions：The abnormal expression of bFGF may play roles in tumor angiogenesis and invasiveness of pituitary adenomas.