鼻咽癌患者放疗前后血清EB病毒特异性DNA酶抗体的变化

Changes of serum Epstein-Barr virus-specific DNaes antibody level in pre- and postradiotherapy nasopharyngeal carcinoma patients

目的：了解鼻咽癌(NPC)患者放疗前后血清EB病毒特异性DNA酶(EBV-DNase)抗体的变化,以评估其预后. 方法：用Western Blot及放射免疫法检测24例NPC患者放疗前后血清EBV-DNase抗体,并用免疫酶法测定血清EB病毒壳抗原IgA(EBVCA-IgA). 结果：①用Western Blot检测NPC患者放疗前后血清EBV-DNaes抗体,放疗前抗体阳性率87.50%(21/24),放疗后为29.16%(7/24),差异显著(P<0.01)；②放免法测得两组血清抗酶率(AER)平均值分别为74.02%、31.75%,二者有显著差异(P<0.05)；③放疗后的血清EBVCA-IgA平均几何滴度(GMT)为1∶23.4,较放疗前明显降低(P<0.05). 结论：PNC放疗后血清EBV-DNase抗体水平有明显下降,比EBVCA-IgA检测更加准确地反映了病情变化.提示血清EBV-DNase抗体检测对评估NPC预后、疗效监测可能有帮助.

Objectives:To evaluate the predictive value of detection of serum Epstein-Barr virus DNase(EBV-DNase) antibody via observing the ch anges of serum EBV-DNase antibody in pre- and postradiotherapy nasopharyngeal carcinoma(NPC) patients. Methods:Serum EBV-DNase antibody in 24 NP C patients pre- and postradiotherapy were detected by Western Blot and immunor adiological method. Their EBVCA-lgA antibody were monitored. Results:EBV-DNase positive rate, AER, EBVCA-lgA geometric mean titer (GMT) of pre- and postradiotherapy sera from 24 NPC patients were 87.50%,74.02%,1∶ 67.6 and 29.16%,31.72%,1∶23.4.The differences between pre- and pos tradiothe rapy were significant( P <0.01, P <0.05, P <0.05). Conclusions:Detection of serum EBV-DNase antibody may be helpful in estimating the progno sis of NPC.