大鼠卵巢冻存前后自体移植的实验研究

Subcutaneous autologous ovarian transplantation in rat before or after cryopreservation

目的：为模拟在体卵巢释放激素的生理功能,将有活力的卵巢组织切除、冻存并自体移植于皮下,观察其移植效果,为临床卵巢移植提供实验依据. 方法：将成熟SD雌性大鼠卵巢切除,部分立即自体移植,部分冻存后自体移植,通过观察阴道脱落细胞变化、子宫重量,移植物的组织学形态及测定血清雌激素(E2)水平,了解移植鼠体内激素分泌状况,判断其内分泌功能. 结果：新鲜移植组：阴道脱落细胞9/10,显示有雌激素分泌,子宫的重量及血清E2水平明显优于去势组(P<0.05),移植卵巢表面有微血管形成,组织切片可见不同发育阶段的卵泡；冻存卵巢移植组：阴道脱落细胞8/10,显示有雌激素分泌,子宫的重量及血清E2水平明显高于去势组(P<0.05),与新鲜移植组无显著差异(P>0.05),移植卵巢表面有微血管形成,组织切片可见发育卵泡. 结论：无论是新鲜还是冻存的卵巢移植,移植后都能分泌雌激素,作用于靶器官维持其功能,为临床卵巢移植提供了实验依据.

Objectives:To imitate the physiological function of hormo ne released by ovaries, active ovarian tissue was removed, cryopreserved and sub cutaneous autotransplanted to see the effect of transplantation. Method s: Autotransplantation was performed immediately after oophorectomy and a we ek later with the left ovary frozen preserved. To study the hormone secretion of the transplanted ovary and to judge their endocrine function, changes of vagina l exfoliative cells and uterine weights, graft histology and the level of serum E2 were determined. Results:In the immediate autotransplantation group, 9/10 of the exfoliative cells secrete Estrogen, and both the growth of th e target organ and the level of E2 were better than those of castrate group ( P <0.05),the capillary network on surface of grafts was profund. Tissue slice : there were many developing follicles of different stages, In frozen ovarian au totransplantation group, 8/10 of the vaginal exfoliative cells secrete estrogen while the growth of the target organ and the level of E2 were better than thos e of castrate group( P <0.05), they were not different significantly from the immediate autotransplantation group( P <0.05),and there were also profound cap illary network on the surface of the grafts. Tissue slice: there were many devel oping follicles. Conclusions:Both immediate autotransplanta tion and frozen ovary group resumed their endocrine function, which provided experimenta l basis for clinical ovarian tissue transplantation.