聚半乳糖醛酸酶高产菌的产酶条件研究

Optimization of conditions for polygalacturonase production by Aspergillus aryzae C491

通过单因素及正交试验对米曲霉 (Aspergillusoryza)C4 91产生聚半乳糖醛酸酶的发酵条件进行了优化。该菌株的摇床发酵滤液以桔子果胶为底物时酶活力可达 3443.8U/mL。产酶最适培养基组成为 :麦芽汁 (糖度 6 % )中添加 6 %桔皮粉 ,2 %硫酸铵 (w/v)。最适培养条件为 :起始pH 4 (灭菌前 ) ,30℃ ,2 5 0r/min ,培养 112h。Tween 80可以促进C 4

Through single factor and orthogonal experiments, the conditions for the extracellular polygalacturonase production by Aspergillus oryzae C491 were optimized. The optimal medium for enzyme production was: malt extract (including malt sugar 6%)with 6% citrus peel powder and 2% (NH 4) 2SO 4(w/v). The optimal cultural conditions were: initial pH 4.0,30℃,250 r/min for 112 hours. Tween 80 could stimulate enzyme production. The enzyme activity of fermentation broth was 3443.8 U/mL.