EMSA法分析STAT5信号分子与周期蛋白cyclin D1的相互作用

Analysis the Interaction of STAT5 Signal Molecular and Cyclin D1 with EMSA

利用蛋白凝胶电脉迁移率变动分析法 (EMSA)分析STAT5信号分子与周期蛋白cyclinD1的相互作用。探针位于cyclinD1启动子区的 - 2 48到 - 2 2 0之间 ,该区域包含SATAT5与cyclinD1的结合序列TTN5AA。STAT5与cyclinD1共形成a、b两条蛋白滞后带 ,当用点突变的探针作用时 ,仅剩下滞后带a。

In this study we describe the interaction between STAT5 signal molecule and cyclin D1 with EMSA method. The probe we used is between-248 to-220 of cyclin D1 promoter and in this region contain the consensus DNA binding sequence for SATA1,3,5-TTN5AA. The interaction of SATA5 with cyclin D1 form two lagged stripes, they are stripe a and stripe b. When using the dot mutagenesis probe to hybrid with nuclear extract and to show the unspecific stripe,only stripe a is left, therefore,stripe b must be the specific stripe in the interaction of the STAT5 with cyclin D1.