短间隔连续部分肝切除对大鼠生存和肝组织结构的影响

EFFECTS OF THE SHORT INTERVAL SUCCESSIVE PARTIAL HEPATECTOMY ON RAT SURVIVAL AND LIVER TISSUE STRUCTURE

在部分肝切除 ( partialhepatectomy ,PH)后的细胞激活 (G0～G1)期 ( 4h)、有丝分裂高峰期 ( 3 6h)及以两者交叉方式进行连续部分肝切除 (successivepartialhepatectomy ,SPH) ,观察其对大鼠生存和肝组织结构的影响。结果表明 ,大鼠对短间隔 (间隔 4和 /或 3 6h)连续部分肝切除的耐受极限取决于各次切除的肝量和间隔时间两个因素 ;连续部分肝切除引起的肝组织结构紊乱程度与部分肝切除次数正相关 ;细胞核数、有丝分裂指数与短间隔连续部分肝切除次数和方式显现复杂的相关性。依SPH中大鼠成活率、肝组织结构变化、生理生化变化为依据 ,确立了 4组 (E、G、K和M组 )适合研究肝再生分子机理的短间隔连续部分肝切除模型 (shortin tervalsuccessivepartialhepatectomy ,SISPH)。

In the paper, we analyzed 4 types of short interval successive partial hepatectomy (SISPH) models (E,G,K and M) were established after the possibility of that were analyzed with 12 types of SISPH models. The effects of SISPH on rat survival and its liver tissue structure were observed with histological method.According the results of A～D models, when the amount of liver once resected is up to 46%,75% or 85%, the survival rates of rat (Rattus norvegicus) were above 75%, but when the amount of liver once resected was up to 92 5%, the rats were all dead after 24 hours. Among the eight types of SISPH models (E～M), lobus externalis sinister resected in first time, lobus centralis sinister and lobus centralis resected in second time, lobus dexter sinister resected in third time and lobus caudatus sinister resected in forth time, and E (3 times of liver resection, interval time between resections was 4, 4 and 4 hours, respectly in turn), G (3 times of liver resection, interval time between resections was 36, 4 and 4 hours, respectly in turn), K (4 times of liver resection, interval time between resections was 36, 36, 36 and 36 hours, respectly in turn) and M models (4 times of liver resection, interval time between resections was 4, 36 36 and 36 hours respectly in turn) were selected out, according the survival rate and interval time, to proceed histological analysis. SISPH could affect the liver on the histological changes, and among that,the changes of hyperplasia of liver and mitosis of chymohepatocytes were, like in E model, most conspicuous. Almost all the hepatocytes of remanent liver lobes becames hyperplasia,and the mitotic index was 15 times of the control.The sharp hyperplasia and quick proliferation gave rise to a series of changes of the liver structure, like obvious confusion of the structure of liver lobes and narrowing of the liver sinusoid.In the other hand, the percentages of binuclear hepatocytes of all the hepatoctes might also be affected by the SISPH, but there were much distinct among the 4 models. The results showed that the amount of the liver once resected might not exceed 85% of the whole liver, and the sustain limit of rat to SISPH is dependent on the amount of liver resected and the interval time between successive partial hepatectomy. It was found that the models as represented by groups E, G, K and M, can be considered as useful models for studying the change of liver structure, the rate of rat survival in SISPH as well as the molecular mechanism of rat liver regeneration.