摘要
用Tween-80非离子型胶束增溶竹红菌甲素、乙素(HA、HB)及金丝桃蒽酮(HYP)等醌类光敏剂 ,发现该胶束对HA的基态和激发态都有保护作用。相对于含水有机体系 ,HA在胶束中基态的 pKa值升高 ,荧光量子产率增大 ,光敏反应产生的活性中间体1O2 和·OH的产额增加 ,从而提高了其光敏活性 ,这在光敏损伤作用中具有重要的生物学意义。实验还发现 ,Tween-80胶束对HB及HYP的保护作用与HA类似,而且保护作用与醌的结构有关 。
In this work, hypocrellins A and B (HA, HB), and hypericin (HYP) were dispersed in Tween-80 micelle. It was found that the micelle had protective effect on ground state and excited state of HA. Compared with HA in water-contained organic solvents, the pigment in micelle had higher pKa value and higher fluorescence quantum yield. Moreover, the quantum yield of active species produced from photosensitization, 1O2 and ·OH, increased as well. As a result, the photosensitivity of HA was enhanced. The protective effect of Tween-80 micelle on HB and HYP was similar to that on HA, and the protective effect was related to the molecular structures of perylenequinonoid photosensitizers. This provided information for screening better photosensitizers for photodynamical therapy.
出处
《生物物理学报》
CAS
CSCD
北大核心
2001年第4期761-766,共6页
Acta Biophysica Sinica
基金
国家自然科学基金重点项目(39830090)
