阿尔茨海默病相关转基因小鼠模型的构建

Construction of Transgene and Transgenic Mice of Amyloid Precursor Protein Gene with Swedish Mutation

将人类淀粉样前体蛋白瑞典型突变基因 (APPSW)与血小板衍生的生长因子 α链 (PDGFα)的顺式调控因子结合 ,构建成 PDGF- APPSW转基因 ;并通过原核显微注射法将之导入 C5 7小鼠受精卵内 ,成功地获得PDAPSW转基因小鼠模型 ,共出生 2 2只仔鼠 ,其中 5只基因组内有

An amyloid precursor protein gene with Swedish mutation was linked to the regulatory region of platelet derived growth factor α chain to construct a transgene(PDAP SW ).Transgenic mice models of PDAP SW were prepared by means of pronuclear microinjection.Five out of 22 newborn mice were found transgenic positive.