压片法检查蓝藻液泡

Determination of Vacuoles from the Blue-green Algae by Squash Method

鱼腥藻 71 2 0 (Anabaena sp.PCC71 2 0 )在含 0 .1 mol/L Na Cl的条件下培养 4d,90 %以上细胞液泡化。若在正常条件下培养 ,1个多月之后部分细胞开始液泡化 ,随着培养时间延长 ,液泡化细胞比例逐渐提高。液泡化藻丝材料在玻片上经手指轻轻施压 ,细胞破裂 ,液泡从细胞破裂处释出。所释放液泡完全透明 ,大小不等 ,可在相差显微镜下显示 ,个别液泡可弹至细胞外远处。无机盐诱导的液泡化和细胞衰老引起的液泡化之间具有明显的平行性。

The blue-green alga Anabaena sp. PCC 7120 was grown in the medium containing 0.1 mol/L NaCl for 4 d and over 90% of cells became vacuola- ted. If they were grown in the common condition for over month, only a part of cells began vacuolation. The cultures were dropped on slide and a filter paper was put on the cover slip to absorb the surplus water. A light pressure on the cover slip was made by a thumb to break some vacuolated cells. Some vacuoles were released from the vacuolated cells. The vacuoles were fully transparent, varied in size, they have not be showed under general microscope but under phase contrast microscope. Two kinds of cell vacuolations by salt inducing or by aging have parallelism.