孕酮对缺血/再灌注大鼠脑皮层水肿的影响

EFFECT OF PROGESTERONE ON CEREBRAL CORTEX EDEMA IN RATS EXPOSED TO FOCAL ISCHEMIA/REPERFUSION

目的 :探讨孕酮 (progesterone ,PROG)对脑水肿的影响。方法 :48只大鼠随机分为 6组 :即缺血 /再灌 (I/R)组 ,二甲基亚砜 (DMSO)组 ,预防 (pretreatment)组 ,防治 (pre +posttreatment)组 ,治疗 (posttreatment)组 ,地塞米松 (DEXA)组。采用大鼠局灶性脑缺血 /再灌注 (I/R)模型 ,测定大脑中动脉阻塞 (MCAO) 2 4h后脑皮层水、钠、钾、钙含量。结果 :与DMSO组相比 ,应用PROG预防及防治组均能明显降低缺血皮层的H2 O(P <0 .0 1)、Na+ (P <0 .0 1)、Ca2 + (P <0 .0 1)含量 ,升高K+ (P <0 .0 1)含量 ,而治疗组虽能明显降低H2 O(P <0 .0 5 )、Na+ (P <0 .0 1) ,但降低Ca2 + (P >0 .0 5 )和升高K+ (P >0 .0 5 )的效果不显著。DEXA组的结果与PROG预防或防治组类似。结论

Aim: To explore the effects of progesterone(PROG) on brain edema in rats. Methods: Forty eight rats were randomly divided into 6 groups that were ischemia/reperfusion(I/R), dimethl sulfoxide(DMSO), and pretreatment, pre+posttreatment,posttreatment with PROG, and dexamethasone (DEXA) groups. The model of focal cerebral ischemia was established in rats by reversible inserting a nylon thread into the anterior cerebral artery. All rats were decapitated at 24 hours after the left middle cerebral artery occlusion (MCAO) respectively. and then brain H 2O, Na +, K +, Ca 2+ contents were determined.Results: Compared with the result in DMSO group, the content of water ( P <0.0l),Na +( P <0.01), Ca 2+ ( P <0.01) was significantly reduced, but K +( P <0.01) was obviously elevated in ischemia cortex in pretreatment group or pre+posttreatmeflt group with PROG. There was also significant reduction in water ( P <0.05) and Na +( P <0.01), but was not significantly changed in Ca 2+ ( P >0.05) and K +( P >0.05) in posttreatment group with PROG. The changes occurring in DEXA grooup were similar to those found in pretreatment group or pre+posttreatinent group with PROG. Conclusion: Pretreatment or pretreatment plus posttreatment with progesterone can significantly reduce brain edema in I/R.