摘要
目的 :研究SH SY5Y神经杂交瘤细胞的钙缓冲能力。方法 :通过膜片钳手段 ,测量未分化的SH SY5Y细胞钙离子通道电流 ;并应用显微荧光测量游离钙离子浓度和高钾去极化的方法 ,研究胞内Ca2 + 浓度上升后浓度恢复的动力学过程。结果 :未分化的SH SY5Y细胞存在钙离子通道电流 ;在刺激时间间隔较短时 (<15 0s) ,胞内钙浓度的恢复过程会由于缓冲机制的饱和而变慢 ;而时间间隔 >15 0s时 ,缓冲物质则可以基本恢复使得胞内钙的恢复过程基本保持不变。结论
Aim: To study the calcium buffer in SH SY5Y cells. Methods: By using patch clamp technique,measure voltage gated calcium currents in undifferentiated SH SY5Y cell line. Then,by using microfluometric technique,detect the intracellular [Ca 2+ ] i and the dynamic recovery course after high K + induced [Ca 2+ ] i elevation. Results: There are voltage gated calcium currents in undifferentiated SH SY5Y cell line. When the time intervals between the stimulus are <150s,the recovery course will be much more deferred because of the saturation of the intracellular calcium buffer; and when the intervals between the stimulus are >150 s,the buffer will recruit so as to lead to the steadiness state of the recover course. Conclusion: Calcium buffer proteins have an important effect in the course of cellular calcium signal transduction.
出处
《中国应用生理学杂志》
CAS
CSCD
北大核心
2001年第4期352-355,共4页
Chinese Journal of Applied Physiology
基金
国家自然科学基金资助项目 ( 6 0 0 710 30
30 0 0 0 0 6 2 )
