棉铃虫单核衣壳核多角体病毒囊膜蛋白odv-e66基因及其邻近区域的序列分析

Sequence Analysis of Helicoverpa armigera Single-nucleocapsid Nucleopolyhedrovirus odv-e66 Gene

杆状病毒ODV E6 6蛋白是包涵体来源病毒 (occlusion derivedvirus ,ODV)囊膜的结构蛋白 ,ODV囊膜对ODV的稳定性和感染性具有重要作用。本文报道了HaSNPVodv e6 6基因及其邻近区域共 4 2 37bp的核苷酸序列及其分析结果。HaSNPV的odv e6 6基因编码区全长 2 0 19bp ,推测编码一个由 6 72个氨基酸残基组成的 ,分子量为 74 .5kD的蛋白质。在起始密码子ATG上游具有杆状病毒晚期转录起始信号ATAAG。与其他杆状病毒ODV E6 6的氨基酸序列比较分显示HaSNPVODV E6 6蛋白具有多个保守区域 ,包括N末端的强疏水功能区、序列中部的一个可能的核定位信号RKIW ,两个Leu zipper以及 5个跨膜区。odv e6 6基因上游的两个ORF分别与AcMNPV的orf10 8和orf10 9具有同源性 。

ODV E66 is the major protein of occlusion derived virions envelope of baculovirus.An odv e66 gene of Helicoverpa armigera single nucleocapsid nucleopolyhedrovirus was identified and sequenced.The HaSNPV odv e66 gene had a size of 2 109 bp and potentially coding a protein of 672 amino acids,with a molecular weight of 74.5 kD.A late transcriptional motif,ATAAG,was found at 20～ 24nt uppstream of the translational start codon ATG.There was a TATA box,a GATA box and a CAGT motif at 13nt, 92nt, 141nt upstream of ATG,respectively.Amino acid sequences alignment with so far identified baculovirus'ODV E66 had revealed a conserved hydrophobic region at the N terminal of ODV E66 proteins.A potential nuclear locating signal RR(K)IW was found conserved in all ODV E66 proteins.Two leu zipper motifs were found in the middle of ODV E66 proteins.Six potential transmembrane regions were found in HaSNPV ODV E66 protein.Two of them were conserved in the other baculoviruses'.Analysis with computer shown that there are several putative glycosylation sites and phosphorylation sites in HaSNPV's ODV E66protein.These indicated that odv e66 was a conservative gene in baculovirus.Primary analysis of Ac109 homologue, Ac108 homologue and p13 has been done with computer.A strong hydrophobic region was found in HaSNPV Ac108 homologue protein.This region is conserved in baculovirus.