棉铃虫核多角体病毒iap2基因的克隆和序列分析

Cloning and Sequence Analysis of the iap2 Gene of Helicoverpa armigera Single-nucleocapsid Nucleopolyhedrovirus

棉铃虫核多角体病毒 (Helicoverpaarmigerasinle nucleocapsidnucleopolyhedrovirus,HaSNPV)基因组中发现了一个抗细胞凋亡基因iap2。该基因位于病毒基因组的BamHI F片段 ,全长 75 3个核苷酸 ,编码 2 5 0个氨基酸 ,预计蛋白质分子量 2 9.2kD。在iap2基因上游 - 30～ - 33的位置有一个TATA框 ,在 5 0～ 5 3的位置有一个早期转录信号CAAT ,在终止密码子下游有一个poly(A)信号AATAAA ,HaSNPViap2基因编码一个锌指结构和两个BIR结构。与LdMNPV、AcMNPV、SeMNPV和OpMNPV的IAPs及果蝇DIAP2和人类XIAP等九种IAPs相比较 ,HaSNPVIAP2与其它的BIR(baculovirusIAPrepeater)和RING结构在Cys/His基元 (motif)

An iap 2 gene of Helicoverpa armigera single nucleocapsid nucleopolyhedrovirus was identified.It was localized Bam H I F fragment of the genome.Nucleotide sequencing showed the open reading frame was 753nt,encoded 250 amino acids with a predicted size of 29.2kD.A'TATA'box was found-30～-33nt upstream of the translational start codon ATG.There is an early consensus translational motif CAAT,-50～-53nt upstream of the translational start codon ATG.A typical poly(A)signal was identified 46～51nt downstream of the translational stop codon TAA.HaSNPV iap 2 gene encode one typical RING and two BIRs.The alignment of AcMNPV?OpMNPV?LdMNPV?LdMNPV?SeMNPV?DIAP and XIAP indicated that they had consensus BIR motifs and RING zinc finger motif.It was suggested HaNPV IAP2 had a potential anti apoptotic function.