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hDaxx与细胞肿瘤抑制子p53在体内外的相互作用 被引量：9

INTERACTION OF HUMAN hDaxx WITH p53 in vivo AND in vitro
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摘要 与急性早幼粒细胞性白血病蛋白 (promyelocyticleukemiaprotein ,PML)在体内相互作用共定位于细胞核PML致癌结构域 (PMLoncogenicdomains,PODs)的人Daxx(humanDaxx ,hDaxx) ,能结合Fas死亡结构域诱导细胞凋亡。细胞肿瘤抑制子p5 3抑制细胞及病毒转录 ,提高细胞内Fas的表达并调节细胞凋亡。为了探索hDaxx与 p5 3在诱导细胞凋亡中有无相互作用及其作用效果 ,利用酵母双杂交体系测定发现p5 3通过C端与hDaxx结合 ,共免疫沉淀反应及Westernblot结果显示hDaxx与 p5 3能在体内外直接结合。hDaxx与 p5 Human Daxx (hDaxx), a novel transcriptional repression protein, interacts and colocalizes with promyelocytic leukemia protein (PML) in PML oncogenetic domains (PODs). The hDaxx binding the death domains (DD) of Fas induces apopotosis. Cellular tumor suppressors p53 inhibits the transcription of cells and virus. The p53 enhances the expression of Fas and mediates apopotosis. In order to probe the interaction and effect of hDaxx with p53, hDaxx was interacts with p53 by its C terminus 62 amino acids using yeast two hybrid assay. Western blot showed that hDaxx binds to p53 in vivo and in vitro by co immuno precipitation reaction. Combination and interaction of hDaxx with p53 may mediate cellular cycles.
作者 谭立志 万艳平 吴移谋 刘传爱 余敏君 尹卫国 廖端芳
机构地区 南华大学基础医学院病原生物学研究所 南华大学基础医学院药物药理学研究所
出处 《微生物学杂志》 CAS CSCD 2001年第4期32-34,共3页 Journal of Microbiology
基金 国家教育部高等学校骨干教师资助计划 ( 2 0 0 0 6 5 )
关键词 HDAXX P53 相互作用 肿瘤抑制子 hDaxx, p53, interaction.
分类号 R730.54 [医药卫生—肿瘤]
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参考文献8

  • 1[1]Yang, X., L., Khosravi-Far, R., Chang, H., Y., et al. Daxx, a novel Fas-binding protein that activates JNK and apopotosis[J].Cell, 1997,89:1067 ～ 1076.
  • 2[2]Ishov, M., A., Sotnikov, G., A., Negorev, D., et al. PML is critical for ND10 formation and recruits the PML-interacting protein Daxx to this nuclear structure when modified by SUMO-1 [J].J. Cell Biology, 1999,147: 221 ～ 233.
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同被引文献23

  • 1蒋永林,王静,王鑫,余敏君,万艳平.人乳头瘤病毒16型E6蛋白真核表达载体的构建及表达[J].南华大学学报（医学版）,2006,34(4):505-508. 被引量：4
  • 2高慧,黄正芳,王琴,李厚达,毕志刚.人乳头瘤病毒16 E6/7转基因小鼠模型的建立[J].中华皮肤科杂志,2007,40(5):286-288. 被引量：4
  • 3Liu Anyuan, Wan Yanping, Tan Lizhi, et al. Down-regulation of cytokine secretion and repression of apoptosis hDaxx in macrophages. Chinese Journal of Cancer Research, 2005, 17 ( 1 ): 44-48.
  • 4Lin Zhenhua, Yemelyanova AV, Gambhira R, et al. Expression pattern and subcellular localization of human papillomavirus minor capsid protein L2. Am J Pathol, 2009, 174( 1 ): 136-143.
  • 5Tang Qiyi, Maul GG. Mouse cytomegalovirus immediate-early protein 1 binds with host cell repressors to relieve suppressive effects on viral transcription and replication during lytic infection. J Virol, 2003, 77(2): 1357-1367.
  • 6Mendoza-Villanueva D, Diaz-Chavez J, Uribe-Figueroa L et al. Gene expression profile of cervical and skin tissues from human papillomavirus type 16 E6 transgenic mice. BMC Cancer, 2008, 8: 347.
  • 7Young - Gyu, Young - Sun Kang, Heoyoung - Park, et al. Apoptosis signal- regulating kinase 1 control the proapoptic function of death- associated protein(Daxx) in the cytoplasm[ J ]. The Journal of Biological Chemistry, 2001,276(42) :39103 - 39106.
  • 8Lukas Cermak, Sarka, Simova, et al. Molecular mechanisms involved in CD3 - mediated apoptosis of TF - 1 cells[J]. The Journal of Biological Chemistry, 2002,277(10) :7955 - 7961.
  • 9Pascal Lopez, Frederique, Vidal, et al. Gene control in germinal differentiation: RNF6, a transcription regulatory protein in the mouse sertoli cell[J]. Molecular and Cellular Biology, 2002,22(10) :3488 - 3496.
  • 10Ishov AM, Vladimirova OV, Maul GG. Daxx- mediated accumulation of human cytomegalovirus tegument protein PP71 at ND10 facilitates initiation of viral infection at these nuclear damain[J]. Journal of Virology, 2002,76(15) :7705 - 7712.

引证文献9

二级引证文献17

微生物学杂志
2001年 第4期

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