淋病奈瑟菌粘附性质粒的研究

STUDY ON ADHERENCE PLASMIDS IN Neisseria gonorrheae

从 1株临床分离的淋病奈瑟菌 (N .gonorrheae,Ng)分别检测出耐氨苄青霉素 (AMPr)及四环素 (TCr)的 2种质粒 (R质粒 ) ,其分子量分别为 2 5 .2Mu和 4.5Mu。进一步研究发现 ,编码TCr 的质粒也编码普通菌毛 ,因而与其粘附性有关。消除此R质粒不但细菌的TCr 消失 ,电镜下观察其菌毛几乎全部消失 ,其粘附性也显著降低 ,故此R质粒也被确认为是粘附性质粒 (Adh质粒 )。

Two R plasmids encoding with tetracycline resistance TC r of MW 25.2 Mu and 4.5 Mu ampicillin resistance AMP r were detected from a Neisseria gonorrheae strain N.g 141 respectively. The R plasmids were found to encode ordinary pili as well as adherence of bacteria to urinary epithelia in further study. After curing the plasmids both of TC r and adherence disappeared, observation under electromicroscope no pili were found. These indicated that the R plasmids were adherence plasmids.