三个STR位点在哈萨克族中的遗传多态性(英文)

Genetic Polymorphism of D16S539, D7S820 and D13S317 Loci in the Kazak Ethnic Population Using Multiplex Amplification

运用复合 PCR扩增 ,6 %变性聚丙烯酰胺凝胶电泳结合银染技术对我国新疆 10 2位无关的哈萨克族个体进行 D16 S5 39,D7S82 0 ,D13S317的 STR位点的调查 ,为建立新疆哈萨克族群体数据库提供资料 .经统计学检验 ,3个位点的基因型频率分布符合 Hardy- Weinberg平衡定律 .结果显示 3个位点的期望杂合度为 :0 .9439、0 .935 6、0 .930 4,累积 PIC=0 .990 5 ,DP=0 .9998,PE=95 72 .此外 ,比较新疆哈萨克族与其他 4个人群的等位片段频率 ,发现除与北京汉族在 D7S82 0位点上无统计学意义外 (P>0 .0 5 ) ,其他均可见显著性差异(P<0 .0 5 ) .同时 ,在 8个家系 42人的调查中无一突变发现且均按孟德尔遗传规律传递 . 3个

One hundred and two unrelated individual from Chinese Kazak Ethnic population in Xinjiang were investigated at three short tandem repeats (STR) loci (D16S539, D7S820, D13S317) using multiplex amplification, 6% denaturing PAGE and silver staining. The population genetic date of the distribution of allele frequencies and genotype frequencies on all three loci were obtained and were in accordance with Hardy Weinberg equilibrium. The expected heterozygosities for these loci were 0.943 9, 0.935 6 and 0.930 4 with the cumulated PIC= 0.990 5 , DP= 0.999 8 , PE= 0.957 2 .Significant difference was found in comparison with other populations. In the sample of 8 families ( n =42) no new mutations could be found. It suggests that this multiplex amplification of three STR loci can be used in forensic identification and genetic research of population as well.