摘要
目的:探讨注射用头孢呱酮钠细菌内毒素的检查法。方法:同时采用凝胶法和终点显色基质法。结果:当样品浓度大于2.5mg/ml时,对两法均显示不同程度的抑制作用,当样品浓度小等于2.5mg/ml时,对两法均无干扰。结论:在注射用头孢哌酮钠细菌内毒素的检查中,最佳浓度≤2.5mg/ml,而采用终点显色基质法能定量测定内毒素的含量。
The detection of bacteria endotoxin in Cefoperazone Sodium for Injection has been carried out with the methods of gel-clot test and end-point chromogenic assay,simultaneously. The result shows that,the inhibition occurs in different degree in both two methods as the sample concentration is more than 2. 5 mg/ml,while no any interference effect is found as the concentration less than or equal to 2. 5 mg/ml. It indicates that,in the test of bacteria endotoxin in Cefoperazone Sodium,the best concentration is less than or equal to 2. 5mg/ml. The method of end-point chromogenic assay can exactly detect the concentration of endotoxion in the drug.
出处
《中国药品标准》
CAS
2001年第3期56-58,共3页
Drug Standards of China
关键词
注射用头孢哌酮钠
细菌内毒素检查法
凝胶法
特点显色基质法
抑制作用
Cefoperazone Sodium for Injection
Bacteria endotoxion test
Gel-clot test
End-point chremogenic assay
Inhibition
