荧光定量聚合酶链反应对乙型肝炎患者个体用药疗效的评定

The accidence exploration of estimation of curative effect in hepatitis B by fluorescence quantitative PCR

应用荧光定量聚合酶链反应 (FQ- PCR) ,对 5 0例乙肝患者连续检测血清中乙型肝炎病毒 (HBV)的数量 ,观察临床个体用药治疗的效果。结果有 41例随着治疗的进程 ,血清中 HBV- DNA相应地逐渐减少 ,最后降至阴性 ,另有 9例连续治疗半年后 HBV- DNA无任何下降趋势。认为 FQ- PCR可以检测 HBV的真实感染和复制情况 ,对于乙型肝炎的临床诊断。

To measure the number of hepatitis B virus (HBV) in serum and observe the clinical curative effect,a method of Fluorescence quantitative PCR(FQ PCR)was used to measure the contents of HBV DNA in serum before and after the treatment by using fluorescence quantitative PCR kits. Results showed there were 41 persons whose HBV DNA contents in serum dropt off and became negative at last.The other 9 patients′ HBV DNA contents in serum had no downtrend after 6 months′ treatment. These suggest FQ PCR can be used to monitor the true state of HBV infection and amplification. It is significant for the clinical diagnoses of HBV, for the selection of treatment scheme and for the estimation of curative effect.