马鹿茸促进表皮细胞和软骨细胞分裂的新多肽(英文)

A NEW POLYPEPTIDE PROMOTING EPIDERMAL CELLS AND CHONDROCYTES PROLIFERATION FROM CERVUS ELAPHUS LINNAEUS

目的 研究马鹿 (CervuselaphusLinnaeus)茸多肽的化学结构和促进细胞有丝分裂的生物活性。方法 用酸性水提取鹿茸多肽 ,多肽组分经过CM SepharoseFastFlow和SephadexG 5 0柱色谱分离后 ,再用反相HPLC (C6)纯化。结果 从马鹿茸得到一个单一多肽化合物。结合激光解析电离飞行时间质谱 ,N 端Edman降解和氨基酸分析结果表明 ,该多肽是由 32个氨基酸残基组成的直链多肽 ,分子量 32 16u ,主要含缬氨酸、丙氨酸、赖氨酸和甘氨酸 ,无半胱氨酸 ,其一级结构氨基酸排列顺序为 :N端 VLSAADKSNVKAAWGKVGGNAPAFGAEALLRM ,与已知蛋白序列同源性远小于 5 0 %。VAPP促进大鼠表皮细胞 (浓度 0 4 - 5 0mg·L-1)和家兔肋软骨 (浓度 10 - 5 0mg·L-1)细胞增殖。

AIM To investigate the chemical structure of velvet antler polypeptide from red deer ( Cervus elaphus Linnaeus) and the cell proliferation promoting activity. METHODS The polypeptide was isolated with acid water extraction and the final purification of this material was accomplished by column chromatography on CM Sepharose Fast Flow, on Sephadex G 50, then by repeated reversed phase HPLC (C 6). RESULTS Velvet antler polypeptide (VAPP) consists of a single linear chain of 32 amino acid residues. Its structure was determined by a combination of MALDI TOF MS analyses, N terminal Edman degradation and amino acid analyses. Amino acid sequence of VAPP was: N terminus VLSAADKSNVKAAWGKVGGNAPAFGAEALLRM. VAPP at 0 4-50 and 10-50 mg·L -1 showed marked stimulant effects on the growth of rat epidermal cells and rabbit costal chondrocytes, respectively. CONCLUSION Velvet antler polypeptide is a new polypeptide promoting cells proliferation.