心肌肌钙蛋白I荧光免疫测定的临床意义

The clinical significance of cardiac Troponin I measurements with radial partition immunoassay

目的 :为评价扩散分离免疫技术 ,荧光免疫定量测定心肌肌钙蛋白I(troponinI,TnI)的临床价值。方法 :我们测定了 6 0例健康成年献血员和 30例非心肌梗死血清肌酸磷酸激酶 (CK)增高者清晨空腹血清TnI,观察了 10例急性心肌梗死患者血清TnI及其它心肌损伤标志物的动态变化。结果 :健康成年献血员TnI 95 %可信上限 0 8μg/L ;非心肌梗死CK增高者中 6例CK MB也增高 ,无 1例TnI超过正常上限者 ;急性心肌梗死患者胸痛发作后 3～ 6hTnI开始增高 ,12～ 2 4h达峰值 ,16 8h后仍有 8例患者TnI高于正常。结论 :TnI是目前特异性的心肌损伤标志物 ;TnI测定对判断心肌损伤与否有重要意义。

Objective:To evaluate the clinical significance of cardiac troponin I (TnI) measurements with radial partition immunoassay Methods:Of the cases ( n =100) studied, 60 were healthy adult blood donor,30 had the elevations of total creatine kinase (CK) and 10 had acute myocardial infarction The cases were evaluated clinically by ECG TnI and CK MB were determined with specific monoclonal monoclonal sandwich assay by Deda Stratus II analyzer Results:The 95% upper reference limit was 0 8μg/L of TnI in the healthy adult blood donor Among the cases with elevations of CK, six patients had elevation of CK MB,but none had increased TnI The levels of TnI were elevated only in 10 patients with acute myocardial infarction In these cases TnI levels rose above the reference range within 3～6 hours, the peak value in 12～24 hours, and remaining elevated in 8 cases for 168 hours following the onset of chest pain Conclusion:Cardiac TnI is a highly specific biochemical marker for myocardial injury The measurement of TnI should facilitate distinguishing whether elevations of CK are due to myocardial or skeletal muscle injury