偶然分枝杆菌形态变化的超微结构的研究

ELECTROMICROSCOPE-STUDY OF THE MORPHOLOGICALCHANGES OF M.FORTUITUM IN CULTURE

目的 探讨偶然分枝杆菌超微结构变化与功能关系。方法 取在血平板上培养 1～ 6天的临床分离株培养物 ,并以标准株作对照 ,用电镜观察其超薄切片。结果 随着培养时间的延长 ,菌体形态从长杆状向短杆状至球状变化 ;同时菌体拟核区由细长逐渐增粗至模糊不清 ,二分裂也随之变为不可见 ;胞质中脂质小空泡从多见至少见 ;菌体末端的成堆糖元颗粒由可见变至未见 ,而异染颗粒从无变至 1～ 3个。结论 菌体形态结构变化与培养基微生态环境的改变相关。

Aim To a research on the function and change in ultrastructure of M.fortuitum.Method Clinical strains cultured on the blood agar plate for six days was taked to observe its ultrathin with electron microscope and contrast with standard strains.Result With the extending of culturing time,the long rod of germs shape has been changed into short rod then into sphero-rodlike.Germ in nucleoid is growing into thick and dim one from thin one.The second division become invisible as it changes.Lipid vocuole in cell can be found to be more,then little,A crowded of glucogon granule in germs end can be found then become invisible.There is one or three metachromatic granule growing out from nothing.Conclusion Changes in germs structure relate to medium in environment.