人-猪链球菌感染性综合征的病原特征分析

ANALYSIS ON PATHOGENIC FEATURE OF SYNDROME INFECTED BY S.SUIS FROM HUMAN AND SWINE

目的 评价人源和猪源猪链球菌的药敏和致病力情况 ,分析这些菌株间的同源性。方法 用K -B法对这些菌株进行药敏试验 ;用菌株对家兔和小白鼠进行攻击试验 ;用菌体脂肪酸分析和随机DNA基因扩增分析技术进行菌株间的同源性研究。结果 人源和猪源猪链球菌对青霉素钾、氯霉素、头孢拉定、头孢呋新、头孢三嗪、头孢噻甲羧肟、菌必治、万古霉素、氨苄青霉素、红霉素敏感 ,对四环素、链霉素不敏感 ,小白鼠对这些菌株不敏感 ,而家兔敏感 ,接种这类菌株可致家兔死亡。菌体脂肪酸分析和随机DNA基因扩增分析提示 :这些菌株间的以及与猪链球菌 2型参考菌株间的亲缘关系很近 ,与血链球菌、无乳链球菌等 7种链球菌及肠球菌的亲缘关系较远。结论 这些人源和猪源猪链球菌均为猪链球菌 2型 ,致病力极强 ,人源与猪源猪链球菌同源。

Aim To evaluate the drug sensitivity and pathogenicity of S.suis from human and swine,to analyse the homogeneity of the strains of S.suis.Methods Drug sensitive test were carried by K-B;attack test were carried on rabbit and rat by S.suis;Study of homogeneity were carried through by Thalli fatty acid profile analysis and randomly amplified polymorphic DNA analysis.Results S.suis from human and swine were sensitive to potassium penicillin,chloramphonicol,cefradine,cefuroxime,ceftriaxone,cefotaxime,rocephin,vancomycin,ampicillin,erythromycin,but not sensitive to tetracycline and streptomycin.Rat was not sensitive to the strains,but rabbit was sentive and went die after inoculated with S.suis.Thalli fatty acid profile analysis and randomly amplified polymorphic DNA analysis showed:the relationship among the strains were close,so between the strains and reference S.suis serotype 2, but the relationship between the strains and other 7 kinds of streptococcus,such as S.sanguis,S.Agalactiae etc,and with entorococcus were far. Conclusion All the strains of S.suis from human and swine are confirmed to be S.suis serotype 2, there pathogenicity are strong,homogeneity exist among S.suis from human and swine.