摘要
目的 研究阿克他利 (Acta)对小鼠Ⅱ型胶原性关节炎 (CIA)的治疗作用。方法 采用Ⅱ型胶原 (CⅡ )乳剂皮内注射诱导的小鼠CIA模型。在此基础上 ,检测小鼠足肿胀、免疫功能的改变 ,以及对CⅡ的迟发性变态反应 (DTH)和血清中抗CⅡ抗体的测定 ,同时进行了病理组织学的检查。结果 Acta(10 ,30 ,90mg·kg-1)ig对CⅡ诱导的小鼠足肿胀有明显的抑制作用 ;体外研究发现 ,Acta(10 ,30 ,90mg·kg-1)能使CIA小鼠过高的ConA增殖反应和IL 2的产生恢复至接近正常 ,同时对CIA小鼠过高的IL 1产生有明显的抑制作用 ;Acta(10 ,30 ,90mg·kg-1)ig可以明显减轻CⅡ诱发迟发性变态反应 (DTH) ,但Acta对CIA小鼠体内的抗体的产生无显著影响。病理学检查表明 ,Acta(10 ,30 ,90mg·kg-1)ig可以减轻CIA小鼠的骨膜增生和软骨破坏。结论 Acta对CIA小鼠具有治疗作用 ,该作用可能是通过细胞免疫调节实现的。
OBJECTIVE To study the therapeutic action of actarit(Acta) in type Ⅱ collagen\|induced arthritis(CIA) mice. METHODS To prepare CIA mice model by CⅡ emulsion and to observe the change of inflammatory and immune function in CIA mice treated with Acta.RESUITS Acta (10,30,90 mg·kg -1 ×40 d) intragastric injection(ig) inhibited inflammatory swelling,multiple arthritis, pathologic change in ankle of CIA mice. In CIA mice,the high response of splenocytes, the increased IL 2 synthesis of CIA mice and the elevated IL 1 synthesis of PMΦ were all reduced to normal by treated with Acta(10,30,90 mg·kg -1 ).Acta (10,30,90 mg·kg -1 ×38 d) significantly suppressed the delayed type hypersensitivity (DTH) reaction induced with type Ⅱ collagen (CⅡ) in CIA mice. But Acta (10,30,90 mg·kg -1 ×40 d) had no effect on the production of anti CⅡ antibodies in CIA mice.CONCLUSION Acta showed therapeutic action in CIA mice which could be related to modulate cell mediate immunity.
出处
《中国药学杂志》
CAS
CSCD
北大核心
2001年第12期814-817,共4页
Chinese Pharmaceutical Journal
