TNF-α反义寡核苷酸对犬颅脑爆炸伤后颅内压的影响

EFFECTS OF ANTISENSE OLIGONUCLEOTIDES DIRECTED AT TNE-α ON THE INTRACRANIAL PRESSURE IN DOGS FOLLOWING CRANIOCEREBRAL EXPLOSIVE INJURY

为探讨TNF α反义寡核苷酸对颅脑爆炸伤后致伤犬颅内压的影响。将 18只犬随机分为人工脑脊液组 (ACSF)、正义链组和反义链组 ,利用已建成的颅脑爆炸伤模型装置致伤 ,伤前经小脑 延髓池分别注入人工脑脊液、TNF α正义寡核苷酸和TNF α反义寡核苷酸 ,比较 3组动物脑组织含水量和颅内压的变化。与人工脑脊液组和正义链组相比 ,伤前经小脑 延髓池脑内局部应用TNF α反义寡核苷酸组 ,颅脑爆炸伤后脑组织内含水量明显减少 (P <0 0 5 ) ,颅内压明显降低 (P <0 0 5 )。提示TNF α反义寡核苷酸可以减轻颅脑爆炸伤后脑水肿 ,降低颅内压。

To investigate the effects of antisense oligonucleotides directed at TNF-α on the intracranial pressure following craniocerebral explosive injury. Eighteen dogs were randomly divided into artificial cerebrospinal fluid (ACSF) group, sense group and antisense group. Using the model of craniocerebral explosive injury, ACSF, the sense oligonucleotides to TNF-α and the antisense oligonucleotides to TNF-α were administered into cerebellomedullar cisterna before injury respectively in three groups, and then the intracranial pressure and contents of brain H 2 O in the bilateral hemispheres in three groups were compared. As compared with the ACSF groups and the sense groups, the contents of brain H 2 O in the antisense groups were significantly reduced( P <0 05) at 6 hours post-trauma in the hemispheres of both sides, and high intracranial pressure following craniocerebral explosive injury was significantly lowered( P <0 05) . The above results strongly suggested that antisense oligonucleotides directed at TNF-α could attenuate intracranial pressure following craniocerebral explosive injury.