影响红细胞冻干效果的因素分析

ANALYSES OF THE EFFECTS OF LYOPHILIZATION ON RBC RECOVERY

探讨了几个相关因素对红细胞冻干回收率的影响。红细胞来自健康人静脉血 ,ACD抗凝 ,对红细胞进行预处理 ,冰冻干燥 16h ,再水化。结果表明 ,预处理 2组红细胞的回收率明显高于预处理 1组 ,分别为 81%和 45 % ;冻干体系中含高和中浓度葡萄糖 ,细胞回收率分别为 ( 4 7 2± 10 5 ) %和 ( 4 5 1± 10 7) % ,两组差异无显著性意义 (P >0 0 5 ) ,但与低浓度葡萄糖组有显著性差异 ;再水化缓冲液渗透压对红细胞冻干回收率也有一定的影响 ,高、中、低 3种再水化缓冲液条件下红细胞回收率差异有显著性意义 (P <0 0 1)。

Lyophilized storage of red blood cells(RBC) may provide a useful storage alternative Both shelf life and transportation logistics can be improved over those for existing refrigerated or frozen storage. The present study was designed to evaluate the effects of lyophilization process on mean RBC recovery. Blood was drawn from healthy adult having no history of either hemoglobinophathy or abnormal RBC metabolism. Blood stored in plastic transfer bags were lyophilized for 16 hours, then it was rehydrated . In group 1, samples were stored at 4℃ for 6 hours, while in group 2 were stored at 4℃ for 5 hours, then 25℃ for 1 hour. The mean RBCs recovery in group 2 was higher than group 1(81 4±7 6% vs 62±10 2%,respectively). Samples were lyophilized using buffer containing different concentrations of glucose, and studies showed that there was no significant difference in mean RBCs recovery between samples in lyophilization buffer containing high and medium concentration of glucose, but low concentration of glucose in buffer resulted in lower mean RBCs recovery. The mean RBCs recovery was 30% in low concentration of glucose buffer and 45 1%, 47 2% in medium and high concentrations of glucose buffer, respectively. We also observed effects of different rehydration buffer with different osmotic pressure, and it showed that high osmotic pressure buffer resulted in higher mean RBCs recovery than iso-osmotic buffer. There was little change in the cell size and cell volume. The results presented in this report clearly demonstrate that human RBCs can be successfully lyophilized. Lyophilization buffer and rehydration buffer may influence the mean RBCs recovery.