一氧化氮对急性肝功能衰竭大鼠生存的影响

The effect of NO precursor or NOS inhibitor on survival of rats with acute liver failure

目的 探讨一氧化氮 (NO)前体及一氧化氮合酶 (NOS)抑制剂对急性肝功能衰竭 (ALF)大鼠生存的影响。方法 切除大鼠 90 %肝脏制备ALF模型 ,选用NO前体或NOS抑制剂诱导或抑制机体产生NO ,观察在ALF时NO对大鼠肝、肺、肾、肠等重要脏器功能及生存期的影响。结果 应用NO前体 ,ALF大鼠丙氨酸转氨酶 (ALT)显著下降 ,肝、肺、肾、肠等重要脏器组织病理损害明显减轻 ,大鼠 2 4h、72h生存率明显提高 ;NOS抑制剂使ALT明显升高 (P <0 0 5 )。结论 NO能够减轻ALF大鼠肝、肺、肾、肠等重要脏器组织结构和功能的损伤 ,有利于ALF大鼠生存 ;而NOS抑制剂则加重脏器组织结构和功能的损伤。

ObjectiveTo observe the e ff ect of NO precursor or/and NOS inhibitor on the survival of acute liver failure( ALF) rats.MethodsModel of ALF rat was established by resecting 90% of the rat liver and the effect of NO prec ursor or/and NOS inhibitor was observed.Resu ltsAdministration of NO precursor significantly improved the liver, lung, kidney and bowel function. The rats′ survival rate at 24 h, and 72 h increased significantly, whereas NOS inhibitor deteriorated fu nctions of important organs(P<0.05).Conclusio nNO precursor alleviates the damage of the ALF rats′ tis sue structure and function of important organs, leading to an increased survival , NOS inhibitor aggravates the damage of the functions of important organs, and endangers the rat′s survival.