白桦提取物解热作用及对下丘脑组织中5-HT、NE、DA含量影响的实验研究

Study Antipyretic Effect of Betula Plaatyphylla Suk(BPS) Leaves Extract—Effect on 5-HT、NE、DA in Hypothalamus of Rats

目的 :研究白桦提取物对酵母致大鼠发热的影响及对下丘脑组织中 5 HT、NE、DA含量的影响。为其解热降温机理作用提供依据。方法 :用荧光分光光度计在 365nm、385nm、32 5nm处为激发波长 ,以4 85nm、4 80nm、375nm为发射波长 ,测定 5 HT、NE、DA含量。单位以每mg鲜脑组织含 5 HT、NE、DA的ng数表示。结果 :酵母模型组大鼠体温及下丘脑组织中 5 HT、NE、DA含量均明显高于正常对照组 ,给药组大鼠给予白桦提取物后 1h体温显著下降 ,下丘脑组织中 5 HT、NE、DA含量亦明显降低。结论 :本实验结果显示 ,白桦提取物对酵母致发热大鼠有明显的解热作用 ,其解热作用与影响体温调节中枢介质 5 HT、NE、DA的含量有关。

Objective: That study effect of BPS extract on in febrile rats induced by zymonsa and on 5 HT、NE、DA in hypothalamus of rats, provide a scientific evidence for the antipyretic and foblowing effects mecchanism. Method:Amethood with fluorospectrophotometry was esstablished for the determination of 5 HT、NE、DA, The excitation wavelength was 365nm、385nm、325nm and the emission wavelength was 485nm、480nm、375nm. Result:The temperature and content 5 HT、NE、DA in Hypothalamus of rats in model group are all higher than those in control group. The temperature of rats in drug given group fall remarkbally after being given drug 1 hour 5 HT、NE、DA in Hypothalamus fall remarkbally also. Conclusion: Bps extract have antipyretic effect in febrile rats induced by zymosa and its antipyretic effect was associated with its reducing the contents of 5 HT and NE and DA in hypothalamus.