针刺对非胰岛素依赖性糖尿病大鼠下丘脑外侧区的作用

Effect of Acupuncture on Lateral Hypothalamic Area of Non Insulin-Dependent Diabetes Mellitus Rat

目的 :了解针刺对非胰岛素依赖性糖尿病 (NIDDM )的中枢神经作用机制。方法 :采用神经电生理和生化技术观察针刺对NIDDM大鼠空腹血糖 (FBS)、空腹胰岛素FINS)、C 肽 (C P)、胰岛素敏感性指数(IAI)和下丘脑外侧区 (LHA)神经细胞自发放电频率及其神经递质含量的影响。结果 :NIDDM大鼠FBS、FINS、C P和LHA神经细胞自发放电频率及其去甲肾上腺素 (NA)、多巴胺 (DA)含量明显高于正常大鼠 ,而IAI和LHA中 5 HT含量明显低于正常大鼠。针刺后NIDDM大鼠FBS、FINS、C P和LHA神经细胞自发放电频率及NA和DA的含量均明显回降 ,而IAI和LHA中 5 HT水平明显回升。LHA神经细胞自发放电频率与FBS、FINS、C P水平呈正相关。结论 :针刺对NIDDM大鼠LHA的调整作用可能是纠正其胰岛素抵抗 (IR) ,调整其异常内分泌代谢的一个重要环节。

To study the central nervous mechanism of acupuncture in non insulin dependent diabetes mellitus (NIDDM). Methods:Neuroelectricphysiology and biochemical technique were used to observe the changes of fasting blood sugar (FBS), fasting insulin(FINS), Cpeptide(C P), insulin sensitivity index (IAI) as well as the frequency of spontaneous discharges of nerve cell and the levels of monoamine neurotransmitters in the Lateral Hypothalamic Area (LHA) of NIDDM rats. Results: The frequency of spontaneous discharges of nerve cell in LHA, the levels of Noradrenaline(NA), Dopamin(DA) in LHA and the levels of FBS、FINS, and C P of NIDDM rats were higher the those in the normal rats, but the level of IAI and content of serotonin (5 HT) in LHA were lower than those of the normal rats. After acupuncture treatment, the levels of FBS, FINS, C P, and the frequency of spontaneous discharges of nerve cell and the levels of NA and DA in LHA were markdely reduced, while the level of IAI and content of 5 HT in LHA were obviously increased. The frequency of spontaeous discharges of nerve cell in LHA has a positive interrelation on the levels of FBS, FINS, and C P. Conclusion:The regulation of acupuncture on LHA of NIDDM rats is possibly one of the important link in the to correect insulin resistance and abnormal endocrine and metabolism.