用蛔虫卵细胞分裂期鉴别生活力的观察

IDENTIFICATION OF VITAL CAPACITY OF ASCARIS EGG DURING ITS MITOTIC TIME

目的用蛔虫卵细胞分裂期鉴别生活力。方法采用蛔虫卵平皿滤膜培养法。结果蛔虫卵培养后的第4 d,发育为 4细胞分裂期 ;第 5d为多细胞期 ;第 6d为桑椹期。结论变异数分析表明蛔虫卵发育过程稳定 ,在此段时间内观察蛔虫卵的形态 ,卵细胞易于辨认 ,操作者易于掌握 。

Objective To identify vital capacity of ascaris egg during 4 6 days (mitotic time) instead of conventional 30 days (infective stage) after the egg being cultured in plate. Methods Plate culture method with double layers of filter membrane, i.e. one layer of filter paper covered with one layer of plastic filter membrane. Results Ascaris eggs were identified 4, 5, 6, 7, 15 and 30 days after being cultured, and coefficient of variation analysis showed that during all cultured stage the development of eggs was stable, i.e. there were no statistic differenences among them. However, the morphology of eggs 4 days (4 cell stage), 5 days (multicell stage), and 6 days (morula stage) after being cultured was easy to be identified. Conclusions For death dignosis of ascaris egg, it is correct enough to identify the morphology of egg 4, 5, 6 days after being cultured in plate.