芦苇愈伤组织对渗透胁迫和ABA的若干生理响应

Some Physiological Response of the Calluses from Reed Ecotypes to Osmotic Stress and Exogenous ABA

研究了沙生芦苇胚性愈伤组织 (SREC)和水生芦苇胚性愈伤组织 (WREC)对PEG 60 0 0的胁迫及外源ABA的生理响应。结果显示 :10 %～ 30 %的PEG胁迫使 2种愈伤组织的游离脯氨酸(Pro)含量升高、质膜透性增大、提高了保护酶 (SOD、CAT、POD)的活性 ,降低了细胞相对含水量 (RWC) ,SREC的游离Pro含量和保护酶活性的升高幅度大于WREC ,质膜透性和RWC的变化前者小于后者。

We've studied the physiological changes of the embryogenic calluses of sand reed(SREC) and the water reed(WREC) in response to the osmotic stress of PEG 6000 and exogenous ABA.The results showed that the contents of free proline,relative membrane permeability and safeguard enzyme(CAT,POD and SOD)activity of SREC and WREC increased under the PEG osmotic stress treatment from 10%～30%,while the relative water content(RWC) decreased under the same treatment.Still the contents of proline and safeguard enzyme activity of SREC increased higher than these of WREC While the relative membrane permeability and RWC of SREC decreased less than these of WREC.It also showed the response of WREC to exogenous ABA was much higher than that of SREC.