摘要
对 4种类胡萝卜素的高效液相色谱 (HPLC)测定方法进行比较 ,选出了适合本实验室的测定方法 :分析柱为Nova pakC18(3 9mm× 30 0mm) ,以乙腈和三氯甲烷 (92∶8)为流动相 ,检测波长为 4 70nm和 4 5 0nm双波长 ,并采用该方法对胡萝卜、南瓜、羽衣甘蓝 3种蔬菜的数百份样品进行了α 胡萝卜素、β
Four different methods were compared to determine the carotinoids with high performance liquid chromatography.The optimal method was selected,which adopted Nova pak C 18 (3 9 mm×300 mm)column and acetonitrile∶trichloromethane(92∶8) mobile phase.The contents of α carotene and β carotene in carrots,pumpkins and kales were determined by this method.
出处
《华北农学报》
CSCD
北大核心
2001年第3期92-97,共6页
Acta Agriculturae Boreali-Sinica
