与枣核性状相关联的RAPD标记的筛选

Identification of RAPD Markers Linked to the Seed Character of Chinese Jujube

用 ３４０个随机引物，对枣有核池和无核池进行了扩增，共扩增出 ３ ３４８条 ＤＮＡ带，选出 １１个多态性引物，进一步用这１１个引物进行个体验证，发现只有引物 Ｓ１５４在金丝小枣的 １６个类型中（占供试有核类型 ８８．２１％）扩增出一条８２０ ｂｐ的特异带，而无核小枣的６个类型中（占供试无核类型８５．７１％）无此特异带。综合分析初步认为，Ｓ１５４８２０是与枣有核性状相关的分子标记，该标记需进一步验证。

The seed pool and seedless pool were analysed by using random amplified polymorphic DNA(RAPD). 340 primers were screened, and a total of 3 348 DNA bands were amplified. 11 polymorphic primers were identified, and each forms was verified by using the 11 polymorphic primers. Only primer S154 amplified a 820 bp DNA band on 16 forms of Jinsixiaozao, but the DNA band was not amplified on 6 forms of Wuhexiaozao.This results showed that S154820 was a molecular marker linked to the seed character.